Protocol Summary:	Equilibrate Cellulose
			Add salt to RNA
			Denature and bind to cellulose
			Remove, denature and bind again
			wash cellulose 4 times in high salt
			wash once in low salt
			Elute twice

65-70 C heat source 1 mg of Total RNA

Prepare Resin:

• Remove oligo-dT and buffers from fridge and equilibrate to RT.
• Spin oligo-dT aliquot in microfuge, 10 sec. (2000 to 5000 x g)
• Remove sup.
• Add 200 ul Wash Buffer, mix thorooughly.
• Spin 10 sec
• Remove sup.

• Prepare 1 mg of Total RNA in 450 ul TE
• Add 50 ul 5 M NaCl
• Denature at 65 C, 5 minutes
• Chill on ice
• Add RNA to oligo-dT Mix thoroughly
• Incubate 5 min. at RT with gentle agitation
• Spin 10 seconds
• Remove Sup to original tube
• Repeat Denaturation and binding
• Spin 10 sec
• Remove sup to original tube, store on ice
• Add 400 ul Wash Buffer
• Agitate by hand to resuspend beads
• Transfer to column reservoir with 1 ml pipet
• Incubate 2 min with gentle agitation
• Spin 10 sec
• Remove sup to new tube
• Repeat wash 3 times
• Add 400 ul Low Salt Buffer
• Spin 10 sec
• Take column to new tube
• Add 200 ul warm Elution Buffer
• Incubate 2 min with gentle agitation
• Spin 10 sec
• Repeat elution with 200 ul fresh Elution Buffer
• Incubate 2 min with gentle agitation
• Spin 10 sec