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Enzyme Recognition Site
NEBuffer
OPA Buffer System
0.5X   1X   2X   Pref
 Unique Buffers
Cla1
 		 AT^CGAT
 
NEB #4
35  50  100  M 
  
BamH1
 
 
 
 		 G^GATCC
 
 
 
 
NEB BamH1
85  100  100  H

 
 
  
	150 mM NaCl
	 10 mM Tris-HCl pH 8
	 10 mM MgCl 
	  1 mM DTT
	100 ug/ml BSA
Bgl II
 		 A^GATCT
 
NEB #3
<25  50  100  H
  
Dra1
 		 TTT^AAA
 
NEB #4
 85  100   65  L
  
EcoR1
 
 
 		 G^AATTC
 
 
 
EcoR1
 65  85   100  H

 
 		 
	50  mM NaCl
	100 mM Tris-HCl pH 7.5 
	10  mM MgCl
EcoRV GAT^ATC
NEB #2
<25  65  100  H
  
HindIII A^AGCTT
NEB #2
<30  100  100   M
  
Pst1 CTGCA^G
NEB #3
75   100   100   M
  
Sal I
 
 
 
 		 G^TCGAC
 
 
 
 
NEB #Sal I
<<25  <<25  100  H

 
 
  
	150 mM NaCl
	 10 mM Tris-HCl pH 8
	 10 mM MgCl 
	  1 mM DTT
	100 ug/ml BSA
SmaI CCC^GGG
NEB #4
35  100   100   KL
  
XmaI C^CCGGG
NEB #1
100   100   100   L
  

New England BioLabs Buffer Scheme
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4 
10 mM Bis Tris Propane-HCl
10 mM MgCl
 1 mM DTT
 pH 7.0
 
10 mM Tris-HCl
50 mM NaCl
10 mM MgCl
 1 mM DTT
  pH 7.9
 
 50 mM Tris-HCl
100 mM NaCl
 10 mM MgCl
 1 mM DTT
  pH 7.9
 
10 mM Tris-acetate
50 mM KOAc
10 mM MgOAc
 1 mM DTT
  pH 7.9

Buffer system listed under the NEB buffers is the OPA (One Phor All) system from Pharmacia. Position indicates relative percent activity in different strengths of OPA (0.5X, 1X, or 2X). 

L     10 mM Tris-HCl pH 7.5   10 mM MgCl2  1 mM DTT
M     10 mM Tris-HCl pH 7.5   10 mM MgCl2  1 mM DTT   50 mM NaCl
H     50 mM Tris-HCl pH 7.5   10 mM MgCl2  1 mM DTT  100 mM NaCl
KL    10 mM Tris-HCl pH 8.0   10 mM MgCl2  1 mM DTT   20 mM KCl
KM    10 mM Tris-HCl pH 7.5   10 mM MgCl2  1 mM DTT   50 mM KCl
KH    10 mM Tris-HCl pH 8.0   10 mM MgCl2  1 mM DTT  100 mM KCl
Chris Seidel