Quick Silver Stain Protocol

1. wash gel in 50% Methanol, 10 min.

2. wash gel in 5% Methanol,  10 min.

3. incubate 10 min in DTT Soln (4 ul 1M DTT in 100 mls H20)

4. Rinse gel with H20

5. add 0.1% Silver Solution (0.1g AgNO3 in 100 ml H20) 10 minutes or longer

6. wash 3 times quickly in ddH20 (5-10 sec each)

7. add 1/3 of developing solution, wash briefly (solution may turn yellow)

8. pour off solution and add remaining 2/3 of developing solution

9. develop until bands are developed to satisfaction

10. stop development by adding solid citric acid (monohydrate) until fizzing stops

11. Rinse gel with H20

12. for drying, equilibrate gel in 35% Ethanol, 2% Glycerol

Development Solution   250 ml
  7.5 g NaCO3
  250 ml H20
  125 ul formaldehyde