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and   antibodies   associated   at   body   both   confined   elongation   Elongation   enriched   for   Forms   forms   gene   H8   initiation   largest   necessary   of   over   phospho   phosphorylated   phosphorylation   pol   polymerase   promoter   recognizes   regions   Ser2   Ser5   Serine   serine   subunit   the   to   transcription   transcriptional   unphosphorylated   with  

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RNA polymerase II

Elongation Forms and antibodies

Ab

polymerase

4H8

recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II

phospho Ser2

RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation

phospho Ser5

RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

RNApolII (last edited 2015-09-08 22:17:16 by ChrisSeidel)